Coding

Part:BBa_K2332314:Design

Designed by: Camillo Moschner   Group: iGEM17_UCL   (2017-10-12)


E-cadherin_PhoCl Fusion Protein, a photosensitive cell adhesion protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2550
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 752
    Illegal BamHI site found at 828
    Illegal BamHI site found at 944
    Illegal BamHI site found at 1868
    Illegal BamHI site found at 2170
    Illegal XhoI site found at 1552
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 208
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 65
    Illegal BsaI site found at 465
    Illegal BsaI site found at 872
    Illegal BsaI site found at 1414
    Illegal BsaI.rc site found at 306
    Illegal BsaI.rc site found at 3183


Design Notes

Required PCR out of both template BioBricks in order to overcome the RFC10 stop-codon generation problem.


Source

Fusion of BBa_K2332311 & BBa_K2332312

References